Blood: The Last Vampire is a 2000 anime film produced by Production I.G and SPE Visual Works and directed by Hiroyuki Kitakubo. The film premiered in theaters in Japan on November 18, 2000. A single-volume manga sequel, titled Blood: The Last Vampire 2000 and written by Benkyo Tamaoki, was published in Japan in 2001 by Kadokawa Shoten, and in English by Viz Media in November 2002 under the title Blood: The Last Vampire 2002.
The story is set in 1966. Its main protagonist is a girl named Saya, who hunts bat-like creatures called chiropterans. Saya is introduced on a subway train, where she assassinates a man in a suit. Her American contacts or handlers arrive. One of them, David, begins to brief Saya on another mission, while the other, Louis, discovers that the man Saya has just killed was probably not a chiropteran.
Saya is a Japanese vampire slayer whose next mission is in a high school on a US military base in 1960s Japan, where she poses as a student. She uses a katana/samurai sword to kill vampires.